Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Sphingomyelinase C 2 (sph2), partial

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Cat No.

LISG-001

Description

A recombinant partial sphingomyelinase C 2 protein corresponding to residues 158-433 of the Leptospira interrogans sph2 sequence.

Abbr

sph2

Alias

LA_1029; Sphingomyelin phosphodiesterase 2; SMase 2

Source

Recombinant expression in Escherichia coli

Species

Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)

Applications

Protein characterization, antibody development, and sph2-related biochemical research.

Package

20 μg
100 μg
1 mg

Product Overview

The protein is expressed in Escherichia coli and supplied with an N-terminal 10×His tag and a C-terminal Myc tag for purification and detection.

Form

Liquid or lyophilized powder

Enzyme Commission Number

EC 3.1.4.12

Activity

Not tested

Molecular Weight

39.3 kDa

Purity

≥90% as determined by SDS-PAGE

Structure

Partial protein; expression region 158-433 aa.

Composition

N-terminal 10×His tag
C-terminal Myc tag

Storage

Store at -20°C or -80°C after receipt. Divide into aliquots for repeated use and avoid repeated freeze-thaw cycles. Working aliquots may be kept at 4°C for up to one week.

Buffer

Liquid form: Tris/PBS-based buffer containing 5-50% glycerol.
Lyophilized form: Tris/PBS-based buffer containing 6% trehalose before lyophilization.

Warnings

Avoid repeated freeze-thaw cycles.

Function

Recombinant partial sph2 target protein; enzymatic activity of this preparation has not been tested.

Synonyms

Sphingomyelinase C 2; Sphingomyelin phosphodiesterase 2; SMase 2; LA_1029

Targets

sph2

Endotoxin Level

Not tested

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute lyophilized protein in sterile deionized water to 0.1-1.0 mg/mL. For long-term storage, add glycerol to a final concentration of 5-50%, divide into aliquots, and store at -20°C or -80°C.

Amino Acid Sequence

LSHNVFMLPTNLPRWGNLGHDERAKRISKSDYVKNQDVIVFEEAFDTSARKILLDNLREEYPYQTDVVGRTKKNWDASLGNFRSYSLVNGGVVILSKWPIEEKIQYIFNDSGCGADWFANKGFVYVKINKEGKKFHVIGTHAQSQDQNCSNLGIPNRANQFDDIRNFIYSKNIPKDETVLIVGDLNVIKESNEYYDMISRLNVNEPRYVGVPFTWDAKTNEIAAYYYENEEPVYLDYIFVSKSHAQPPVWQNLAYDPVSKQTWTVSGYTSDEFSDH

Quality

Purity: ≥90% by SDS-PAGE
Activity: Not tested
Endotoxin: Not tested

Formulation

Secreted

Notes

Protein length: partial.
UniProt accession: P59116.
Expression region: 158-433 aa.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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