T4 Endonuclease V (T4 PDG)

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Cat No.

TEVG-0001

Description

T4 Endonuclease V is a bifunctional DNA repair enzyme with DNA N-glycosylase and AP lyase activities. It recognizes UV-induced cis-syn cyclobutane pyrimidine dimers in DNA.

Abbr

T4 PDG

Alias

T4 Pyrimidine DNA Glycosylase; T4 Endonuclease V

Source

T4 phage

Species

T4 phage

Applications

DNA damage studies; single-cell gel electrophoresis (comet assay).

Package

500 U
1,000 U

Product Overview

The enzyme cleaves the glycosidic bond at the 5′ side of a pyrimidine dimer to create an AP site, then cleaves the phosphodiester backbone at that site to form a one-nucleotide gap.

Activity

1 U/µL

Contaminants

Nonspecific DNase: Not detected.
E. coli host genomic DNA: <0.05 copies/U.

Molecular Weight

16.1 kDa

Purity

≥95% by SDS-PAGE

Concentration

1 U/µL

Specificity

Recognizes cis-syn cyclobutane pyrimidine dimers generated by UV irradiation.

Unit Definition

One unit is the amount of enzyme required to convert 0.5 µg of UV-irradiated supercoiled pUC19 DNA to >95% nicked plasmid in a 20 µL reaction after 30 minutes at 37°C. Nicking is evaluated by agarose gel electrophoresis, and the irradiated plasmid contains an average of 3–5 pyrimidine dimers.

Storage

Store at -25°C to -15°C. Shelf life: 1 year.

Function

Removes UV-induced pyrimidine dimers through DNA N-glycosylase activity and cleaves the resulting AP site through AP lyase activity.

Synonyms

T4 PDG; T4 Endonuclease V; Pyrimidine Dimer Glycosylase; T4 Pyrimidine DNA Glycosylase

Substrates

DNA containing UV-induced cis-syn cyclobutane pyrimidine dimers.

Reaction

Pyrimidine-dimer DNA → AP-site DNA + released pyrimidine dimer; AP-site DNA → one-nucleotide DNA gap with cleaved phosphodiester backbone.

Advantages

Bifunctional DNA N-glycosylase/AP lyase activity; protein purity ≥95%; no detectable nonspecific DNase; low residual host genomic DNA.

Components

500 U package:
T4 Endonuclease V (T4 PDG), 1 U/µL: 500 µL
10× T4 Endonuclease V Reaction Buffer: 1 mL

1,000 U package:
T4 Endonuclease V (T4 PDG), 1 U/µL: 1 mL
10× T4 Endonuclease V Reaction Buffer: 2 mL

Usage

For enzymatic analysis of UV-induced DNA damage and comet-assay workflows.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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