T4 β-glucosyltransferase, Recombinant

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Cat No.

NATE-0773

Description

In enzymology, a DNA beta-glucosyltransferase is an enzyme that catalyzes the chemical reaction in which a beta-D-glucosyl residue is transferred from UDP-glucose to an hydroxymethylcytosine residue in DNA. It is analogous to the enzyme DNA alpha-glucosyltransferase. This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases.

Abbr

β-glucosyltransferase, Recombinant

Source

E. coli

Applications

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

Product Overview

T4-phage beta-glucosyltransferase, also known as UDP glucose-DNA ?-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351 (end) with C-terminal His-tag, MW= 41.6 kDa, expressed in E. coli.

Form

aqueous solution, Formulated in 200 mM imidazole and 20% glycerol.

Molecular Weight

mol wt 41.6 kDa

Purity

> 83% (SDS-PAGE)

Storage

−70°C

Synonyms

T4 Beta-glucosyltransferase; DNA beta-glucosyltransferase; T4-HMC-beta-glucosyl transferase; T4-beta-glucosyl transferase; T4 phage beta-glucosyltransferase; UDP glucose-DNA beta-glucosyltransferase; uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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