TB-500 Peptide

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Cat No.

TBGZ-001

Description

TB-500 Peptide is a synthetic peptide corresponding to an active region of thymosin β₄ and is reported to have anti-fibrotic and wound-healing activity associated with inhibition of the Akt signaling pathway and binding to actin.

Appearance

White to off-white solid

Product Overview

The peptide is the N-acetylated sequence Leu-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ). Reported effects include promotion of endothelial cell differentiation, dermal angiogenesis, keratinocyte migration and collagen deposition, together with reduced inflammation.

Form

Solid

Activity

Anti-fibrotic and wound-healing activity

CAS_No

885340-08-9

Molecular Weight

889.01

Purity

99.57%

Storage

Store sealed under nitrogen and protect from moisture and light. Powder: -80°C for 2 years or -20°C for 1 year. In solution: -80°C for 6 months or -20°C for 1 month under the same sealed, moisture- and light-protected conditions.

Preparation Instructions

Select the solvent according to the solubility information. After preparing a stock solution, aliquot it to avoid repeated freeze-thaw cycles. If water is used for the stock solution, dilute it to the working concentration and sterilize by filtration through a 0.22 μm filter before use.

Pathway

Akt signaling pathway; purinergic P2 receptor signaling

Function

TB-500 is reported to support tissue repair and regeneration. Its active fragment Ac-Tβ4₁₇–₂₃ prevents apoptosis, promotes cell survival and angiogenesis, stimulates T-cell proliferation, and shows immunomodulatory activity.

Synonyms

TB500; TB 500; TB-500

Targets

Actin; purinergic P2 receptors

Molecular Formula

C₃₈H₆₈N₁₀O₁₄

Solubility

H₂O: ≥ 200 mg/mL (224.97 mM). The saturation concentration was not determined.

Shipping Conditions

Room temperature; shipping conditions may vary by destination.

In vitro

TB-500 shows tissue-repairing and regenerative activity through Ac-Tβ4₁₇–₂₃. This fragment prevents apoptosis, promotes cell survival and angiogenesis, stimulates T-cell proliferation, and exhibits immunomodulatory activity.
Cell migration assay: PAM212 cells; 15-25 μg/mL; 2 days. TB-500 promoted cell migration and wound healing by enhancing the ATP-induced increase in intracellular Ca²⁺ and activating purinergic P2 receptors.

In vivo

A single 10 mg/dose administration has been described in horse and greyhound studies related to doping detection.
BALB/c mouse skin wound model: 15 μg/30 μL by topical administration once daily for 10 days; accelerated wound healing and showed tissue-repair effects.

Amino Acid Sequence

N-Acetyl-Leu-Lys-Lys-Thr-Glu-Thr-Gln

Quality

Selected batch documentation lists purity 99.98%, assay 99.98%, and ee 99.98%.

Protocol

Stock solution preparation in H₂O (required solvent volume):
1 mM — 1 mg: 1.1248 mL; 5 mg: 5.6242 mL; 10 mg: 11.2485 mL; 25 mg: 28.1212 mL
5 mM — 1 mg: 0.2250 mL; 5 mg: 1.1248 mL; 10 mg: 2.2497 mL; 25 mg: 5.6242 mL
10 mM — 1 mg: 0.1125 mL; 5 mg: 0.5624 mL; 10 mg: 1.1248 mL; 25 mg: 2.8121 mL
15 mM — 1 mg: 0.0750 mL; 5 mg: 0.3749 mL; 10 mg: 0.7499 mL; 25 mg: 1.8747 mL
20 mM — 1 mg: 0.0562 mL; 5 mg: 0.2812 mL; 10 mg: 0.5624 mL; 25 mg: 1.4061 mL
25 mM — 1 mg: 0.0450 mL; 5 mg: 0.2250 mL; 10 mg: 0.4499 mL; 25 mg: 1.1248 mL
30 mM — 1 mg: 0.0375 mL; 5 mg: 0.1875 mL; 10 mg: 0.3749 mL; 25 mg: 0.9374 mL
40 mM — 1 mg: 0.0281 mL; 5 mg: 0.1406 mL; 10 mg: 0.2812 mL; 25 mg: 0.7030 mL
50 mM — 1 mg: 0.0225 mL; 5 mg: 0.1125 mL; 10 mg: 0.2250 mL; 25 mg: 0.5624 mL
60 mM — 1 mg: 0.0187 mL; 5 mg: 0.0937 mL; 10 mg: 0.1875 mL; 25 mg: 0.4687 mL
80 mM — 1 mg: 0.0141 mL; 5 mg: 0.0703 mL; 10 mg: 0.1406 mL; 25 mg: 0.3515 mL
100 mM — 1 mg: 0.0112 mL; 5 mg: 0.0562 mL; 10 mg: 0.1125 mL; 25 mg: 0.2812 mL

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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