Terminal Transferase from Calf, Recombinant

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Cat No.

NATE-1926

Description

Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.

Source

E. coli

Species

Calf thymus

Enzyme Commission Number

EC 2.7.7.31

Activity

42,000 units/mg

CAS_No

9027-67-2

Molecular Weight

58000 daltons

Concentration

20,000 units/ml

Unit Definition

One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol dTTP into acid-insoluble material in a total reaction volume of 50ul in 1 hour at 37°C using d(A)18 as primer.

Storage

at -20°C

Synonyms

DNA nucleotidylexotransferase; terminal deoxyribonucleotidyltransferase; terminal addition enzyme; addase; deoxynucleotidyl terminal transferase; deoxyribonucleic acid nucleotidyltransferase; deoxyribonucleic nucleotidyltransferase; terminal deoxynucleotide transferase; TdT; EC 2.7.7.31; 9027-67-2

"TdT" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0692	Native Calf Terminal Transferase	EC 2.7.7.31	9027-67-2	Calf thymus	Inquiry
EXWM-3242	DNA nucleotidylexotransferase	EC 2.7.7.31	9027-67-2		Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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