Thermostable Archaeal Pyruvate Kinase, ≥80°C

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Cat No.

CEGZ-6417

Description

A recombinant archaeal pyruvate kinase with thermal stability to at least 80°C for high-temperature nucleotide triphosphate regeneration.

Abbr

Source

Recombinant E. coli

Species

Archaeal

Applications

For research and in vitro phosphorylation of ribo- and deoxyribonucleoside diphosphates using phosphoenolpyruvate in nucleotide regeneration workflows.

Product Overview

The enzyme is an archaeal pyruvate kinase that supports quasi-irreversible phosphorylation of (d)NDPs using phosphoenolpyruvate as phosphate donor and can serve as a (d)NTP regeneration catalyst.

Form

Liquid

Enzyme Commission Number

EC 2.7.1.40

CAS_No

9001-59-6

Molecular Weight

51.9 kDa

Specificity

Ribo- and deoxyribonucleoside diphosphates; phosphoenolpyruvate-dependent phosphate transfer.

Thermal stability

Thermostable up to at least 80°C

Storage

-20°C

Synonyms

PK; PEPK; phosphoenolpyruvate kinase; phosphoenol transphosphorylase; fluorokinase; pyruvic kinase; pyruvate phosphotransferase; ATP:pyruvate 2-O-phosphotransferase

Substrates

ADP; phosphoenolpyruvate; (d)NDPs

Reaction

ATP + pyruvate = ADP + phosphoenolpyruvate

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM phosphoenolpyruvate
0.04 g/L enzyme
37°C, 2 h

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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