Thermostable Bacterial Acetate Kinase, 80°C

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Cat No.

CEGZ-6415

Description

A recombinant bacterial acetate kinase with thermal stability up to 80°C and broad nucleotide diphosphate acceptance for phosphate-transfer workflows.

Abbr

AcK

Alias

AckA; AK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro phosphorylation of natural nucleoside diphosphates to the corresponding triphosphates using acetyl phosphate, including ATP regeneration systems.

Product Overview

The enzyme uses acetyl phosphate as the phosphate donor to convert natural ribo- and deoxyribonucleoside diphosphates to their triphosphate forms. Its high-temperature stability supports nucleotide regeneration reactions under elevated-temperature conditions.

Form

Liquid

Enzyme Commission Number

EC 2.7.2.1

CAS_No

9027-42-3

Molecular Weight

46.1 kDa

Specificity

Natural (d)NDPs as phosphate acceptors; acetyl phosphate as donor.

Thermal stability

Thermostable up to 80°C

Storage

-20°C

Synonyms

AcK; acetokinase; AckA; AK; acetic kinase; ATP:acetate phosphotransferase

Substrates

ADP; acetyl phosphate; natural nucleoside diphosphates

Reaction

ADP + acetyl phosphate = ATP + acetate

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM acetyl phosphate
0.04 g/L enzyme
37°C, 2 h

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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