Thermostable Bacterial Adenylate Kinase, 60°C

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Cat No.

CEGZ-6407

Description

A recombinant bacterial adenylate kinase with thermal stability up to 60°C and a defined molecular mass of 25.4 kDa.

Abbr

ADK

Alias

AMPK; myokinase

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro phosphorylation of AMP or dAMP to ADP or dADP at mesophilic-to-moderately elevated temperatures.

Product Overview

The enzyme catalyzes ATP-dependent phosphorylation of AMP and supports the corresponding dAMP reaction. It is a thermostable bacterial preparation distinct from higher-temperature adenylate kinase variants.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.3

CAS_No

9013-02-9

Molecular Weight

25.4 kDa

Specificity

AMP and dAMP

Thermal stability

Thermostable up to 60°C

Storage

-20°C

Synonyms

ADK; AMPK; adenylic kinase; adenylokinase; 5'-AMP-kinase; myokinase; ATP:AMP phosphotransferase

Substrates

ATP; AMP; dAMP

Reaction

ATP + AMP = 2 ADP

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM dATP
0.04 g/L enzyme
37°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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