Thermostable Bacterial Adenylate Kinase, 80°C

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Cat No.

CEGZ-6399

Description

A recombinant bacterial adenylate kinase with thermal stability up to 80°C, suitable for high-temperature nucleotide phosphorylation workflows.

Abbr

ADK

Alias

AMPK; myokinase

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro phosphorylation of AMP or dAMP to the corresponding diphosphate nucleotides.

Product Overview

The enzyme catalyzes ATP-dependent phosphorylation of AMP to ADP and also supports phosphorylation of dAMP to dADP. Its high thermal stability distinguishes it from mesophilic adenylate kinase preparations.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.3

CAS_No

9013-02-9

Molecular Weight

26.4 kDa

Specificity

AMP and dAMP

Thermal stability

Thermostable up to 80°C

Storage

-20°C

Synonyms

ADK; AMPK; adenylic kinase; adenylokinase; 5'-AMP-kinase; myokinase; ATP:AMP phosphotransferase

Substrates

ATP; AMP; dAMP

Reaction

ATP + AMP = 2 ADP

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 50°C)
10 mM MgCl2
5 mM AMP
6 mM dATP
0.04 g/L enzyme
50°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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