Thermostable Bacterial Nucleoside-Diphosphate Kinase, 50°C

inquiry

Cat No.

CEGZ-6409

Description

A recombinant bacterial nucleoside-diphosphate kinase with broad substrate acceptance and thermal stability up to 50°C.

Abbr

NDPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro phosphorylation of nucleoside diphosphates to nucleoside triphosphates.

Product Overview

The enzyme transfers phosphate from ATP to nucleoside diphosphates to form the corresponding triphosphates and supports a broad substrate spectrum.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.6

CAS_No

9026-51-1

Molecular Weight

16.6 kDa

Specificity

Broad substrate spectrum for nucleoside diphosphates.

Thermal stability

Thermostable up to 50°C

Storage

-20°C

Synonyms

NDPK; nucleoside 5'-diphosphate kinase; nucleoside diphosphokinase; nucleotide phosphate kinase; NDP kinase; ATP:nucleoside-diphosphate phosphotransferase

Substrates

ATP; nucleoside diphosphates

Reaction

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GDP
6 mM ATP
0.04 g/L enzyme
50°C, overnight

Usage

Research and in vitro use only.

First Name:

Last Name:

Email *

Phone Number:

Company/Institution:

Country or Region:

Quantity:

Services & Products of Interested *

Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

Products

Online Inquiry