Thermostable Bacterial PPK2 Class III Polyphosphate Kinase, 31.7 kDa

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Cat No.

CEGZ-6412

Description

A recombinant bacterial class III PPK2 polyphosphate kinase with thermal stability up to 50°C and activity toward both nucleotide mono- and diphosphates.

Abbr

PPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro NMP/NDP phosphorylation, ATP regeneration, and polyphosphate-driven nucleotide conversion at temperatures up to 50°C.

Product Overview

This PPK2 class III enzyme uses polyphosphate to phosphorylate both nucleoside monophosphates and diphosphates, can substitute for NMPKs and NDPKs, and may generate nucleotide products containing more than three phosphate groups.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.1

CAS_No

9026-44-2

Molecular Weight

31.7 kDa

Specificity

PPK2 class III; polyphosphate-driven phosphorylation of nucleoside monophosphates and diphosphates. Hyperphosphorylation beyond three phosphate groups may occur.

Thermal stability

Thermostable up to 50°C

Storage

-20°C

Synonyms

PPK; PPK2 class III; polyphosphoric acid kinase; ATP:polyphosphate phosphotransferase

Substrates

Nucleoside monophosphates; nucleoside diphosphates; polyphosphate

Reaction

ADP + (phosphate)n = ATP + (phosphate)n-1

Notes

Can be used for ATP regeneration. Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 50°C)
10 mM MgCl2
5 mM GMP
6 mM polyphosphate
0.04 g/L enzyme
50°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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