Thermostable Bacterial PPK2 Class III Polyphosphate Kinase, 32.8 kDa

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Cat No.

CEGZ-6413

Description

A recombinant bacterial class III PPK2 polyphosphate kinase with a molecular mass of 32.8 kDa and thermal stability up to 50°C.

Abbr

PPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro polyphosphate-driven phosphorylation of nucleotide mono- and diphosphates and ATP regeneration.

Product Overview

The enzyme catalyzes polyphosphate-dependent phosphorylation of both AMP and ADP-type substrates, can substitute for NMPKs or NDPKs, and may produce hyperphosphorylated nucleotide species.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.1

CAS_No

9026-44-2

Molecular Weight

32.8 kDa

Specificity

PPK2 class III; nucleoside monophosphates and diphosphates. Hyperphosphorylation beyond three 5'-phosphate groups may occur.

Thermal stability

Thermostable up to 50°C

Storage

-20°C

Synonyms

PPK2 class III; polyphosphoric acid kinase; polyP kinase; ATP:polyphosphate phosphotransferase

Substrates

AMP; ADP; other nucleoside monophosphates and diphosphates; polyphosphate

Reaction

ADP + (phosphate)n = ATP + (phosphate)n-1
AMP + (phosphate)n = ADP + (phosphate)n-1

Notes

Can be used for ATP regeneration. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM polyphosphate
0.04 g/L enzyme
37°C, 2 h

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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