Thermostable Bacterial Pyruvate Kinase, 80°C

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Cat No.

CEGZ-6418

Description

A recombinant bacterial pyruvate kinase with thermal stability up to 80°C and a defined nucleotide diphosphate preference profile.

Abbr

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro nucleotide triphosphate regeneration using phosphoenolpyruvate, including elevated-temperature kinase workflows.

Product Overview

The enzyme supports phosphate transfer between pyruvate/phosphoenolpyruvate and multiple nucleotide donor/acceptor pairs. In the regeneration direction it shows strongest preference for ADP, dADP, GDP, and dGDP, followed by UDP, with lower activity toward TDP, CDP, and dCDP.

Form

Liquid

Enzyme Commission Number

EC 2.7.1.40

CAS_No

9001-59-6

Molecular Weight

51.9 kDa

Specificity

Substrate preference in the regeneration direction: ADP, dADP, GDP, dGDP >> UDP > TDP, CDP, dCDP. ATP, UTP, GTP, CTP, ITP, and dATP can act as phosphate donors in the reverse direction.

Thermal stability

Thermostable up to 80°C

Storage

-20°C

Synonyms

PK; phosphoenolpyruvate kinase; phosphoenol transphosphorylase; fluorokinase; pyruvic kinase; pyruvate phosphotransferase; ATP:pyruvate 2-O-phosphotransferase

Substrates

ADP; dADP; GDP; dGDP; UDP; TDP; CDP; dCDP; phosphoenolpyruvate

Reaction

ATP + pyruvate = ADP + phosphoenolpyruvate

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 50°C)
10 mM MgCl2
5 mM ADP
6 mM phosphoenolpyruvate
0.04 g/L enzyme
50°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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