Thermostable Bacterial Thymidine Kinase, 80°C

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Cat No.

CEGZ-6397

Description

A recombinant bacterial thymidine kinase distinguished by high thermal stability and a broad nucleoside acceptor profile.

Abbr

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro phosphorylation of thymidine, related nucleosides, and selected nucleoside derivatives to the corresponding monophosphates.

Product Overview

The enzyme transfers phosphate from ATP to thymidine and can also accept deoxycytidine, deoxyguanosine, deoxyinosine, uridine, and deoxyuridine; dGTP can also serve as a phosphate donor. It remains thermostable up to 80°C.

Form

Liquid

Enzyme Commission Number

EC 2.7.1.21

CAS_No

9002-06-6

Molecular Weight

22.1 kDa

Specificity

Thymidine and derivatives; additional acceptors include deoxycytidine, deoxyguanosine, deoxyinosine, uridine, and deoxyuridine. ATP is the primary donor; dGTP can also act as donor.

Thermal stability

Thermostable up to 80°C

Storage

-20°C

Synonyms

TK; 2'-deoxythymidine kinase; ATP:thymidine 5'-phosphotransferase

Substrates

ATP; thymidine

Reaction

ATP + thymidine = ADP + dTMP

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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