Thermostable Prokaryotic (d)CMP Kinase, 80°C

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Cat No.

CEGZ-6400

Description

A recombinant prokaryotic cytidylate kinase that preferentially phosphorylates CMP and dCMP and remains thermostable up to 80°C.

Abbr

CMPK

Alias

dCMPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro ATP-dependent phosphorylation of CMP and dCMP, including elevated-temperature nucleotide synthesis workflows.

Product Overview

The enzyme phosphorylates CMP and dCMP efficiently with ATP as the preferred donor, while UMP and AMP are accepted at much lower rates. dCMP is accepted nearly as efficiently as CMP.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.25

Molecular Weight

26.0 kDa

Specificity

CMP and dCMP are preferred; UMP and AMP are phosphorylated at much lower rates.

Thermal stability

Thermostable up to 80°C

Storage

-20°C

Synonyms

CMPK; dCMPK; prokaryotic cytidylate kinase; deoxycytidylate kinase; deoxycytidine monophosphokinase; ATP:(d)CMP phosphotransferase

Substrates

ATP; CMP; dCMP

Reaction

ATP + (d)CMP = ADP + (d)CDP

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 50°C)
10 mM MgCl2
5 mM CMP
6 mM ATP
0.04 g/L enzyme
50°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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