Thermostable Prokaryotic Guanylate Kinase, 80°C

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Cat No.

CEGZ-6401

Description

A recombinant prokaryotic guanylate kinase with broad nucleotide acceptance and thermal stability up to 80°C.

Abbr

GMPK

Alias

dGMPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro phosphorylation of GMP and related purine monophosphates under standard or elevated-temperature conditions.

Product Overview

The enzyme phosphorylates GMP and dGMP, also accepts AMP and to a smaller extent dAMP, and can use ATP or dATP as phosphate donor.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.8

CAS_No

9026-59-9

Molecular Weight

25.4 kDa

Specificity

GMP and dGMP; also AMP and, to a small extent, dAMP. ATP and dATP can serve as donors.

Thermal stability

Thermostable up to 80°C

Storage

-20°C

Synonyms

GMPK; deoxyguanylate kinase; guanosine monophosphate kinase; 5'-GMP kinase; ATP:GMP phosphotransferase; ATP:(d)GMP phosphotransferase

Substrates

ATP or dATP; GMP; dGMP; AMP; dAMP

Reaction

ATP + GMP = ADP + GDP

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 50°C)
10 mM MgCl2
5 mM GMP
6 mM ATP
0.04 g/L enzyme
50°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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