Thermostable Prokaryotic TMP Kinase, 80°C

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Cat No.

CEGZ-6402

Description

A recombinant prokaryotic thymidylate kinase with thermal stability up to 80°C and activity toward several purine and pyrimidine monophosphates.

Abbr

TMPK

Alias

dTMPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro phosphorylation of dTMP and related nucleotide monophosphates, including high-temperature synthesis workflows.

Product Overview

The enzyme primarily phosphorylates dTMP and also accepts dGMP and AMP, with GMP accepted to a smaller extent. ATP serves as the phosphate donor.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.9

CAS_No

9014-43-1

Molecular Weight

24.2 kDa

Specificity

dTMP; also dGMP and AMP, with lower activity toward GMP.

Thermal stability

Thermostable up to 80°C

Storage

-20°C

Synonyms

TMPK; thymidine monophosphate kinase; thymidylate kinase; deoxythymidine 5'-monophosphate kinase; dTMPK; dTMP kinase; thymidylic acid kinase; thymidylic kinase; ATP:dTMP phosphotransferase

Substrates

ATP; dTMP; dGMP; AMP; GMP

Reaction

ATP + dTMP = ADP + dTDP

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 50°C)
10 mM MgCl2
5 mM dTMP
6 mM ATP
0.04 g/L enzyme
50°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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