Thermostable Prokaryotic UMP Kinase, 80°C

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Cat No.

CEGZ-6403

Description

A recombinant prokaryotic UMP kinase with strong UMP preference, allosteric regulation, and thermal stability up to 80°C.

Abbr

UMPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro phosphorylation of UMP in pyrimidine nucleotide metabolism and high-temperature nucleotide conversion workflows.

Product Overview

The enzyme is primarily specific for UMP, with GMP as a secondary substrate and CMP accepted to a much lesser extent. It is inhibited by UTP and activated by GTP.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.22

CAS_No

9036-23-1

Molecular Weight

26.6 kDa

Specificity

Primarily UMP; secondary substrate GMP and much lower activity toward CMP. Inhibited by UTP and activated by GTP.

Activators

GTP

Thermal stability

Thermostable up to 80°C

Storage

-20°C

Inhibitors

UTP

Synonyms

UMPK; uridylate kinase; uridine monophosphate kinase; ATP:UMP phosphotransferase

Substrates

ATP; UMP; GMP; CMP

Reaction

ATP + UMP = ADP + UDP

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 50°C)
10 mM MgCl2
5 mM UMP
6 mM ATP
0.04 g/L enzyme
50°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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