Thermostable Pyrimidine Nucleoside Phosphorylase, 60°C

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Cat No.

CEGZ-6389

Description

A recombinant bacterial pyrimidine nucleoside phosphorylase with thermal stability up to 60°C and a defined molecular mass of 47.3 kDa.

Abbr

PyNP

Alias

Py-NPase; PYNP

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro use in reversible phosphorolysis and synthesis of pyrimidine nucleosides, including phosphate-detection workflows when a low-phosphate preparation is required.

Product Overview

This enzyme catalyzes reversible cleavage of uridine, thymidine, and 2-deoxyuridine to the corresponding pyrimidine base and pentose-1-phosphate, and an almost phosphate-free double-desalted version is available.

Form

Liquid

Enzyme Commission Number

EC 2.4.2.2

CAS_No

9055-35-0

Molecular Weight

47.3 kDa

Thermal stability

Thermostable up to 60°C

Storage

4°C

Synonyms

PyNP; Py-NPase; PYNP; pynpase; pyrimidine ribonucleoside phosphorylase; pyrimidine-nucleoside:phosphate (2'-deoxy)-alpha-D-ribosyltransferase

Substrates

Uridine; 2-deoxyuridine; thymidine; phosphate

Reaction

Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
2-deoxyuridine + phosphate = uracil + 2-deoxy-alpha-D-ribose 1-phosphate
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate

Notes

A double-desalted, almost phosphate-free version is available for phosphate detection assays. Recombinant protein is produced in E. coli and purified by heat treatment and His6-tag affinity chromatography.

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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