Thermostable Thermococcus kodakarensis AMP Phosphorylase

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Cat No.

CEGZ-6396

Description

A recombinant AMP phosphorylase corresponding to the Thermococcus kodakarensis deoA gene, supplied as an active inclusion-body preparation with high-temperature activity.

Abbr

AMPP

Alias

AMPpase; Tk-DeoA

Source

Recombinant E. coli

Species

Thermococcus kodakarensis

Applications

For research and in vitro studies of nucleoside monophosphate phosphorolysis and alpha-D-ribose 1,5-bisphosphate formation.

Product Overview

The enzyme reversibly converts AMP and phosphate to adenine and alpha-D-ribose 1,5-bisphosphate, also exhibits activity toward CMP, UMP, and GMP, and remains active across a broad 20-80°C temperature range.

Form

Liquid

Enzyme Commission Number

EC 2.4.2.57

Molecular Weight

55.0 kDa (monomer)

Specificity

AMP; also active toward CMP, UMP, and GMP

Thermal stability

Thermostable up to at least 80°C; activity at 20-80°C

Storage

4°C

Synonyms

Tk-DeoA; AMPP; AMPpase; nucleoside monophosphate phosphorylase; AMP:phosphate alpha-D-ribosyl 5'-phosphate-transferase

Substrates

AMP, CMP, UMP, or GMP; phosphate

Reaction

AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate
CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate
UMP + phosphate = uracil + alpha-D-ribose 1,5-bisphosphate

Notes

Supplied as active inclusion bodies. Recombinant protein produced in E. coli and purified by inclusion-body purification.

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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