Transglutaminase from guinea pig liver, Recombinant

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Cat No.

NATE-1247

Description

Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross linking of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.

Abbr

Transglutaminase, Recombinant (Guinea pig)

Source

Sf9 cells

Species

Guinea pig liver

Applications

Transglutaminase has been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington's disease and Alzheimer's disease. Transglutaminase has also been used in a study to develop a nonradioactive dot blot assay for transglutaminase activity.

Form

Lyophilized powder from 5.0 mM Tris, pH 7.5, 0.5 mM DTE and 1 mM CaCl2

Activity

> 1.5 units/mg

CAS_No

80146-85-6

Unit Definition

One unit will catalyze the formation of 1.0 μmole of hydroxamate per minute from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 °C. (L-Glutamic acid γ-monohydroxamate is the standard.)

Storage

at -20°C

Buffer

Resuspend powder in 50 mM Tris, pH 7.6

Warnings

Has a C-terminal 6X His-Tag

Synonyms

transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase

"Transglutaminase" Total Products Page

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Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1737	Transglutaminase 7 from Human, Recombinant	EC 2.3.2.13	80146-85-6	E. coli	Inquiry
NATE-1725	Biotinylated Transglutaminase from Human, Recombinant	EC 2.3.2.13	80146-85-6	Insect cells	Inquiry
NATE-1724	Transglutaminase 1 from Human keratinocyte, Recombinant	EC 2.3.2.13	80146-85-6	Insect cells	Inquiry
NATE-1723	Transglutaminase 1 from Human keratinocyte, Recombinant	EC 2.3.2.13	80146-85-6	E. coli	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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