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type III site-specific deoxyribonuclease
Cat No.
EXWM-3575
Description
This is a large group of enzymes which, together with those now listed as EC 3.1.21.3 (type 1 site-specific deoxyribonuclease) and EC 3.1.21.4 (type II site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC 3.1.23 and EC 3.1.24. They have an absolute requirement for ATP but do not hydrolyse it; S-adenosy-L-methionine stimulates the reaction, but is not absolutely required. They recognize specific, short DNA sequences and cleave a short distance away from the recognition sequence. These enzymes exist as complexes with enzymes of similar specificity listed under EC 2.1.1.72 [site-specific DNA-methyltransferase (adenine-specific)] or EC 2.1.1.73
Form
Liquid or lyophilized powder
Enzyme Commission Number
CAS No.
9075-08-5
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
type III restriction enzyme; restriction-modification system
Reaction
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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