Viral dNMP Kinase, Recombinant

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Cat No.

CEGZ-6406

Description

A recombinant mesophilic deoxynucleoside monophosphate kinase of viral origin with activity toward dTMP, dGMP, and selected nucleotide analogs.

Abbr

dNMPK

Source

Recombinant E. coli

Species

Viral

Applications

For research and in vitro phosphorylation of deoxynucleoside monophosphates and selected nucleotide analogs.

Product Overview

The enzyme converts dTMP and dGMP to their corresponding diphosphates and can also act on some nucleotide analogs. ATP and dATP can serve as phosphate donors.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.13

CAS_No

37278-20-9

Molecular Weight

28.6 kDa

Specificity

dTMP, dGMP, and some nucleotide analogs; ATP and dATP can act as donors.

Storage

-20°C

Synonyms

dNMPK; deoxynucleoside monophosphate kinase; ATP:deoxynucleoside-phosphate phosphotransferase

Substrates

ATP or dATP; dTMP; dGMP

Reaction

ATP + dTMP = ADP + dTDP
ATP + dGMP = ADP + dGDP

Notes

Mesophilic enzyme. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM dTMP
6 mM ATP
0.04 g/L enzyme
37°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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