Products

Enzymes for Research, Diagnostic and Industrial Use

Products
Online Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

24 hour
Promise

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.

Y-27632 2HCl

Cat No.
CEI-0297
Description
Y-27632 can inhibit ROCK with Ki of 140 nM.
CAS No.
129830-38-2
Molecular Weight
320.26
Purity
>99%
Storage
2 years at -20centigrade Powder
Targets
ROCK
Molecular Formula
C14H21N3O*2HCl
Chemical Name
(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide dihydrochloride
Solubility
DMSO 64 mg/mL Water 64 mg/mL Ethanol 2 mg/mL
In vitro
Y-27632, an inhibitor of ROCK (Rho-associated coiled-coil forming protein serine/threonine kinase), can inhibit ROCK by binding to the catalytic site. The inhibition was reversed by ATP in a competitive manner. The Ki value of Y-27632 is at least 20 to 30 times higher than those for two other Rho effector kinases, citron kinase and protein kinase PKN. Y-27632 can abolished stress fibers in Swiss 3T3 cells with 10 uM, without affecting the G(1)-S phase transition of the cell cycle and cytokinesis. Comparing to Y-30141, another ROCK inhibitor, the potency of Y-27632 is 1/10 of that of Y-30141 inhibiting the kinase activity and stress fiber formation. Y-27632 can inhibit migration and morphological change of MM1 cells by suppressing LPA-induced tyrosine phosphorylation of FAK (focal adhesion kinase) and paxillin, which can be regulated by ROCK. Y-27632 dose-dependently inhibited chemotactic and invasive activity of Walker 256 cells s, a rat mammary cancer cell line. Y-27632 also inhibited the phosphorylation level of regulatory myosin light chain in vitro.

Our Products Cannot Be Used As Medicines Directly For Personal Use.

0
Click unfold / close
Inquiry Basket
Delete selected Quote Check Out
Decide to move out of the shopping cart?
Sure No, Back

Please choose product!

< Go Back
You have already added to buy this product