α(2→3,6) Neuraminidase from Clostridium perfringens, Recombinant

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Cat No.

NATE-0972

Description

Releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates.

Abbr

α-Neuraminidase, Recombinant (Clostridium perfringens)

Alias

Sialidase

Source

E. coli

Species

Clostridium perfringens

Applications

Structural analysis of oligosaccharides; Determining sialic acid linkage; Glycoprotein deglycosylation; Removing heterogeneity from glycoproteins

Form

A sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).

Activity

>10 U/ml (>40 U/mg)

Molecular Weight

~41 kD

Specificity

This enzyme releases α2-3, and α2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc α2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5.

Optimum pH

Storage

Store at 2-8°C. DO NOT FREEZE.

Buffer

5X concentrated buffer which when diluted gives 50 mM sodium phosphate pH 6.0.

Synonyms

neuraminidase; sialidase; α-neuraminidase; acetylneuraminidase; exo-α-sialidase

"Neuraminidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1716	Neuraminidase from Microorganism	EC 3.2.1.18	9001-67-6	Microorganism	Inquiry
NATE-1683	Neuraminidase, recombinant	EC 3.2.1.18	9001-67-6		Inquiry
NATE-0479	Native Clostridium perfringens (C. welchii) Neuraminidase Agarose	EC 3.2.1.18	9001-67-6	Clostridium perfringens (C. welchii)	Inquiry
NATE-0480	Native Clostridium perfringens (C. welchii) Neuraminidase	EC 3.2.1.18		Clostridium perfringens (C. welchii)	Inquiry

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