Description
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.
Abbr
β-Lactamase, Recombinant (Pseudomonas aeruginosa)
Species
Pseudomonas aeruginosa
Applications
β-lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β-lactamase is used to study antibiotic resistance and resistance suppression. Product is produced from Pseudomonas aeruginosa and is expressed in E. coli.
Form
Lyophilized powder containing sodium chloride and potassium phosphate.
Enzyme Commission Number
EC 3.5.2.6
Activity
> 20 U/mg (with cephalosporin C); > 400 U/mg (with benzylpenicilin)
Unit Definition
One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in HEPES buffer, pH 7.0 containing 10 μM zinc chloride at 25ºC.
Synonyms
β-lactamase; penicillinase; cephalosporinase; neutrapen; penicillin β-lactamase; exopenicillinase; ampicillinase; penicillin amido-β-lactamhydrolase; penicillinase I, II; β-lactamase I-III; β-lactamase A, B, C; β-lactamase AME I; cephalosporin-β-lactamase; EC 3.5.2.6; 9073-60-3
