β-Acetylglucosaminidase 18A from Bacteroides thetaiotaomicron, Recombinant

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Cat No.

NATE-1291

Description

β-acetylglucosaminidase 18A, is an enzyme from Bacteroides thetaiotaomicron that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. Recombinant BtAcp18A (GH18), purified from Escherichia coli, is a single domain family 18 Glycoside Hydrolase (GH18).

Abbr

β-acetylglucosaminidase 18A, Recombinant (Bacteroides thetaiotaomicron)

Source

E. coli

Species

Bacteroides thetaiotaomicron

Form

35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol.

Enzyme Commission Number

EC 3.2.1.96

CAS_No

37278-88-9

Molecular Weight

50.3 kDa

Purity

>50% by SDS-PAGE

Concentration

1 mg/mL

Specificity

Mammalian high mannose N-glycans (HMNG), such as Man9GlcNAc2

Optimum pH

7.5

Optimum temperature

37 °C

Storage

This enzyme is shipped at room temperature but should be stored at -20 °C.

Synonyms

beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase; N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase

"β-N-Acetylhexosaminidase" Total Products Page

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NATE-1289	β-acetylglucosaminidase 73A from Clostridium perfringens, Recombinant			E. coli	Inquiry
NATE-0934	β-N-Acetylhexosaminidase from Xanthomonas manihotis, Recombinant			E.coli	Inquiry
NATE-1220	β-N-Acetylhexosaminidase from Streptococcus pyogenes, Recombinant	EC 3.2.1.52	9012-33-3	Streptococcus pyogenes M1 GAS SF370	Inquiry
NATE-0780	Native Canavalia ensiformis β-N-Acetylglucosaminidase	EC 3.2.1.52	9012-33-3	Canavalia ensiformis	Inquiry

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