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β-acetylglucosaminidase 73A from Lactococcus lactis, Recombinant

Cat No.
NATE-1290
Description
This enzyme releases non-reducing terminal β1-2, β1-3, β1-4 and β1-6 linked N-acetylglucosamine from complex carbohydrates. When incubated with oligosaccharides at low concentrations (<50 mU/ml) the enzyme can differentiate between GlcNAcβ1-2Man, GlcNAcβ1-4Man and GlcNAcβ1-6Man linkages. Under such conditions, the enzyme cleaves essentially only β1-2 linked GlcNAc, with two provisos. Firstly, β1-2 GlcNAc is not hydrolyzed if the mannose to which it is substituted has a substitution at C-6. Thus, the enzyme is useful for the analysis of tri-antennary oligosaccharides. Secondly, if the β-linked mannose of the conserved pentasaccharide core is substituted with a "bisecting" GlcNAc then only the β1-2 linked GlcNAc linked to mannose on the α1-3 arm is cleaved. At higher concentrations of the enzyme, β1-4 and β1-6 linked GlcNAc may also be hydrolyzed.
Abbr
β-acetylglucosaminidase 73A, Recombinant (Lactococcus lactis)
Source
E. coli
Species
Lactococcus lactis
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Molecular Weight
19.4 kDa
Purity
>90% by SDS-PAGE
Concentration
1 mg/mL
Specificity
Peptidoglycan
Optimum pH
6.8
Optimum temperature
35 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase; N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase

"β-N-Acetylhexosaminidase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1289 β-acetylglucosaminidase 73A from Clostridium perfringens, Recombinant E. coli Inquiry
NATE-0934 β-N-Acetylhexosaminidase from Xanthomonas manihotis, Recombinant E.coli Inquiry
NATE-1220 β-N-Acetylhexosaminidase from Streptococcus pyogenes, Recombinant EC 3.2.1.52 9012-33-3 Streptococcus pyogenes M1 GAS SF370 Inquiry
NATE-0780 Native Canavalia ensiformis β-N-Acetylglucosaminidase EC 3.2.1.52 9012-33-3 Canavalia ensiformis Inquiry
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