β-Agarase I from E. coli, Recombinant

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Cat No.

NATE-1282

Description

Recombinant Agarase I from E. coli for research on agar hydrolysis and enzymatic mechanisms. Perfect for microbiology and molecular biology studies. Creative Enzymes delivers trusted products.

Abbr

β-Agarase I, Recombinant (E. coli)

Source

E. coli

Species

E. coli

Applications

β-Agarase I digests agarose, releasing trapped DNA and producing carbohydrate molecules which can no longer gel. β-Agarase I can be used to purify both large (> 50 kb) and small (< 50 kb) fragments of DNA from gels. The remaining carbohydrate molecules and β-Agarase I will not, in general, interfere with subsequent DNA manipulations such as restriction endonuclease digestion, ligation, and transformation.

Product Overview

β-Agarase cleaves the agarose subunit, unsubstituted neoagarobiose [3,6-anhydro-a-L-galactopyranosyl-1-3-d-galactose] to neoagaro-oligosaccharides.

Form

50 mM Bis Tris-HCl (pH 6.5), 1 mM Na2EDTA and 50% glycerol.

Concentration

1,000 units/ml

Unit Definition

One unit is defined as the amount of enzyme required to digest 200 μl of molten low melting point or NuSieve agarose to nonprecipitable neoagaro-oligosaccharides in 1 hour at 42°C.

Storage

at -20°C

Synonyms

agarase; AgaA; AgaB; endo-β-agarase; agarose 3-glycanohydrolase; EC 3.2.1.81; 37288-57-6

"Agarase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0040	Native Pseudomonas atlantica Agarase	EC 3.2.1.81	37288-57-6	Pseudomonas atlantica	Inquiry
NATE-1293	β-Agarase 16D from Zobellia galactanivorans, Recombinant	EC 3.2.1.81	37288-57-6	E. coli	Inquiry
EXWM-3945	β-agarase	EC 3.2.1.81	37288-57-6		Inquiry

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