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β-Mannosidase from Bacteroides fragilis, Recombinant

Cat No.
NATE-1185
Description
Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.
Abbr
β-Mannosidase, Recombinant (Bacteroides fragilis)
Source
Bacteroides fragilis NCTC 9343
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.25
Activity
5.32 U/mg
CAS No.
9025-43-8
Molecular Weight
100520.1 Da
Purity
> 95 % as judged by SDS-PAGE
Concentration
48.05 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of pNP per minute from pNP-β-D-mannopyranoside (1.11 mM) in 55.6 mM sodium phosphate buffer, pH 6.0, at 25 °C, and using an extinction coefficient of 18000 M-1cm-1 at 410nm.
Optimum pH
6
Storage
Store at 4°C (shipped at room temperature)
Synonyms
β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8

"β-Mannosidase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-0778 Native Helix pomatia β-Mannosidase EC 3.2.1.25 9025-43-8 Helix pomatia Inquiry
NATE-1186 β-Mannosidase from Bacteroides thetaiotaomicron, Recombinant EC 3.2.1.25 9025-43-8 Bacteroides thetaiotaomicron VPI-5482 Inquiry
NATE-1187 β-Mannosidase from Cellvibrio japonicus, Recombinant EC 3.2.1.25 9025-43-8 Cellvibrio japonicus NCIMB 10462 Inquiry
NATE-1188 β-Mannosidase from Cellvibrio mixtus, Recombinant EC 3.2.1.25 9025-43-8 Cellvibrio mixtus Inquiry
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