β-Mannosidase from Bacteroides thetaiotaomicron, Recombinant

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Cat No.

NATE-1186

Description

Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.

Abbr

β-Mannosidase, Recombinant (Bacteroides thetaiotaomicron)

Source

Bacteroides thetaiotaomicron VPI-5482

Form

Supplied in 3.2 M ammonium sulphate

Enzyme Commission Number

EC 3.2.1.25

Activity

10.62 U/mg

CAS_No

9025-43-8

Molecular Weight

96040.16 Da

Purity

> 95 % as judged by SDS-PAGE

Concentration

45.27 U/ml

Unit Definition

One unit is defined as the amount of enzyme required to release 1μmol of D-mannose per minute from ivory nut mannan (5 mg/mL; freshly treated with 10 % sodium hydroxide and neutralised with acetic acid) in 50 mM sodium phosphate buffer, pH 5.6, containing 1mg/mL BSA, at 37°C, and using the method of Miller (1957) to follow reducing sugar liberated at 575 nm.

Optimum pH

5.6

Storage

Store at 4°C (shipped at room temperature)

Synonyms

β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8

"β-Mannosidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0778	Native Helix pomatia β-Mannosidase	EC 3.2.1.25	9025-43-8	Helix pomatia	Inquiry
NATE-1185	β-Mannosidase from Bacteroides fragilis, Recombinant	EC 3.2.1.25	9025-43-8	Bacteroides fragilis NCTC 9343	Inquiry
NATE-1187	β-Mannosidase from Cellvibrio japonicus, Recombinant	EC 3.2.1.25	9025-43-8	Cellvibrio japonicus NCIMB 10462	Inquiry
NATE-1188	β-Mannosidase from Cellvibrio mixtus, Recombinant	EC 3.2.1.25	9025-43-8	Cellvibrio mixtus	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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