Products

Enzymes for Research, Diagnostic and Industrial Use

6-Phosphogluconic Dehydrogenase from Microorganism


Cat No.
NATE-1937
Description
In enzymology, a phosphogluconate dehydrogenase (decarboxylating) (EC 1.1.1.44) is an enzyme that catalyzes the chemical reaction:6-phospho-D-gluconate + NADP+↔ D-ribulose 5-phosphate + CO2 + NADPH. Thus, the two substRates of this enzyme are 6-phospho-D-gluconate and NADP+, whereas its 3 products are D-ribulose 5-phosphate, CO2, and NADPH. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Source
Microorganism
Form
Lyophilized
Enzyme Commission Number
EC 1.1.1.44
Activity
>40 U/mg protein
Contaminants
(as 6PGDH activity = 100 %)
Glucokinase < 0.01 %
Phosphoglucomutase < 0.01 %
Hexose-6-phosphate isomerase < 0.01 %
Glutathione reductase < 0.01 %
Molecular Weight
ca. 132,000
Isoelectric point
ca. 4.5
pH Stability
5.0 - 10.0
Michaelis Constant
(80 mM GIycylglycine buffer, pH 7.5, at 30 °C)
6-Phospho-D-gluconate, 0.95 mM
NAD+, 0.32 mM
Unit Definition
One unit of activity is defined as the amount of 6PGDH that forms 1 μmol of NADH per minute at 30 °C.
Optimum pH
7.0 - 7.5
Activators
Mg2+, Mn2+, Ca2+, K+, Na+
Thermal stability
(50 mM MES-NaOH buffer, pH 6.8, containing 0.5 M KCl)
No detectable decrease in activity up to 40 °C.
Storage
Stable at -20 °C for at least six months
Stabilizers
KCl, MgCl2, Sorbitol, BSA
Inhibitors
Fructose 1,6-bisphosphate, Erythrose 4-phosphate, NADH
Synonyms
6-Phosphogluconic Dehydrogenase; phosphogluconic acid dehydrogenase; 6-phosphogluconic dehydrogenase; 6-phosphogluconic carboxylase; 6-phosphogluconate dehydrogenase (decarboxylating); 6-phospho-D-gluconate dehydrogenase; EC 1.1.1.44; phosphogluconate dehydrogenase; decarboxylating; 9073-95-4
Download Datasheet:
SDS:


“6-Phosphogluconic Dehydrogenase” Total Products Page

Inquiry
Related Products
Catalog
Product Name
EC No.
CAS No.
Source
Price
CatalogNATE-1652
EC No.EC 1.1.1.44
CAS No.
SourceE. coli
CatalogNATE-0796
EC No.EC 1.1.1.44
CAS No.9073-95-4
SourceE. coli
CatalogNATE-0008
EC No.EC 1.1.1.44
CAS No.9073-95-4
SourceSheep liver
CatalogNATE-0009
EC No.EC 1.1.1.44
CAS No.9073-95-4
SourceYeast
+ See More >>
Related Services
Related Protocols
6-PHOSPHOGLUCONIC DEHYDROGENASE -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country or Region:
Company/Institution:
*Products or Services of Interest:
Quantity:
Project Description:
Our products are not intended for direct clinical use!
*Verification Code:
Verification code

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.


Sitemap | Privacy Policy | Terms and Conditions
Copyright ©2011 - 2021 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-516-512-3133
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country or region:
     
0
Click unfold / close
Inquiry Basket
Delete selected Check Out
Decide to move out of the shopping cart??

Sure No, Back
 

Please choose product!

< Go Back
You have already added to buy this product