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Enzyme Activity Measurement of Phosphogluconate Dehydrogenase (NADP+-Dependent, Decarboxylating) Using Spectrophotometric Assays

Creative Enzymes is a pioneer in testing phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating). The advanced equipment and decade-long experiences allow us to deliver test data with high accuracy and reproducibility. Our results are guaranteed with high precision and low variations that meets the industrial high standards.

Phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) belongs to the family of oxidoreductases. It catalyzes the conversion of 6-phosphogluconate and NADP to ribulose 5-phosphate, with the release of carbon dioxide (CO2), and NADPH. It is also named as the follwing:

  • phosphogluconic acid dehydrogenase;
  • 6-phosphogluconic dehydrogenase;
  • 6-phosphogluconic carboxylase;
  • 6-phosphogluconate dehydrogenase (decarboxylating);
  • 6-phospho-D-gluconate dehydrogenase

Phosphogluconate Dehydrogenase (NADP+-dependent, decarboxylating) is the second dehydrogenase of the pentose phosphate pathway (PPP). The pathway mainly contains two parts: an oxidative branch and a non-oxidative, rearrangement branch. The pathway is present in most species and most tissues. The main function of PPP is to produce NADPH and pentose for biosynthetic reactions. NADPH is essential in protecting the organism against oxidative stress and is required for a variety of reductive biosynthetic reactions, particularly lipid production. 6PGDH plays a crucial role in this pathway because it is the very enzyme that produces NADPH along with the production of ribose 5-phosphate, which is the source of nucleotide and nucleic acid synthesis. Due to the important functions it plays in organisms, this enzyme may pave the path to the treatment of some diseases. For example, it has been posited as a potential drug target for African sleeping sickness (trypanosomiasis). More studies have been carrying out on this enzyme, due to its potential in health care and biotechnology. As a result, proper measurement of the enzymatic activity is essential to most research activities. Creative Enzymes has extensive experiences to conduct activity measurement for phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating). Our professional techniques in spectrophotometric measurement guarantee precise quantification.

Enzyme Activity Measurement of Phosphogluconate Dehydrogenase (NADP+-Dependent, Decarboxylating) Using Spectrophotometric Assays Figure: The crystal structure of 6-phosphogluconate dehydrogenase from the protozoan parasite Trypanosoma brucei.
Reference: Christopher Phillips et al. J. Mol. Biol. 1998 282, 667-681

Creative Enzymes’ expertise lies in customization of activity test methods for each client. We have the cutting-edge equipment and experienced team which allow us to maintain a leading position in this field. With the excellent service quality and specialized services, more and more customers and researchers choose us to be their top choice in enzyme activity determination.