Products

Enzymes for Research, Diagnostic and Industrial Use

Chemically modified Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Cat No.
DIA-280
Description
Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction:D-glucose 6-phosphate + NADP+ ↔ 6-phospho-D-glucono-1,5-lactone + NADPH + H+. This enzyme is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).
Abbr
Chemically modified G6PDH, Recombinant (Leuconostoc mesenteroides)
Alias
G6PDH; GPD
Source
E. coli
Species
Leuconostoc mesenteroides
Applications
Use Glucose-6-phosphate Dehydrogenase for the determination of blood glucose or creatine kinase.
Appearance
White lyophilizate
Activity
>30 U/mg lyophilizate
Contaminants
ATPase: <0.02 Creatine kinase: <0.001 Glutamate dehydrogenase: <0.01 Glutathione reductase: <0.001 Hexokinase and GIucose dehydrogenase: <0.05 Myokinase: <0.05 ''NADH oxidase'': <0.02 ''NADPH oxidase'': <0.0005 6-Phosphogluconate dehydrogenase: <0.001 Phophoglucose isomerase : <0.01 Phosphoglucomutase: <0.001 Glucose: <0.3 μg/mg lyophilizate
Molecular Weight
110 kD (2 identical subunits 55,000 D)
Isoelectric point
4.6
pH Stability
5.0-10.0
Michaelis Constant
NAD: 1.4 x 10-4 mmol/l NADP: 3.7 x 10-5 mmol/l Glucose-6-P: 3.7 x 10-4 mmol/l (NAD as coenzyme) Glucose-6-P: 2.0 x 10-4 mmol/l (NAD as coenzyme)
Specificity
G6P-DH is highly specific for glucose-6-phosphate and does not react with fructose-6-P, fructose-1,6-P2 or glucose-1P. 2-Deoxyglucose-6-P is slowly oxidized with NAD (5%) and with NADP (4%).
Optimum pH
7.8
Activators
Phosphate, 5 mmol/l: 100% (NAD), 80% (NADP) Phosphate, 50 mmol/l: 100% (NAD), 80% (NADP) Without Mg2+: 90% (NAD), 80% (NADP) Mg2+, 3 mmol/l: 100% (NAD), 100% (NADP) Mg2+, 30 mmol/l: 100% (NAD), 100% (NADP) HCO3-, 3 mmol/l: 100% (NAD), 100% (NADP)
Thermal stability
Up to +40°C for native G6P-DH, up to +50°C for modified G6P-DH
Stability
At +2 to +8°C within specification range for 18 months. Store dry.
Inhibitors
NADPH is a competitive inhibitor in the NAD-dependent reaction. Unlike the yeast enzyme, myristic acid, dehydroepiandrosterone and palmitoyl CoA do not inhibit.
Synonyms
D-glucose 6-phosphate dehydrogenase; glucose 6-phosphate dehydrogenase (NADP); NADP-dependent glucose 6-phosphate dehydrogenase; 6-phosphoglucose dehydrogenase; Entner-Doudoroff enzyme; glucose-6-phosphate 1-dehydrogenase; G6PDH; GPD; glucose-6-phosphate dehydrogenase
Download Datasheet:


“Glucose-6-phosphate dehydrogenase” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-143
EC No.EC 1.1.1.49
CAS No.9001-40-5
SourceBacillus sp.
CatalogDIA-145
EC No.EC 1.1.1.49
CAS No.9001-40-5
SourceMicroorganism
CatalogDIA-219
EC No.EC 1.1.1.49
CAS No.9001-40-5
SourceBaker's yeast (S. cerevisiae)
CatalogDIA-321
EC No.EC 1.1.1.49
CAS No.9001-40-5
SourceLeuconostoc mesenteroides
+ See More >>
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogCSUB-0275
EC No.206-067-4
CAS No.298-83-9
Source
CatalogCSUB-0276
EC No.217-488-8
CAS No.1871-22-3
Source
CatalogCSUB-0277
EC No.
CAS No.127615-60-5
Source
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1898
EC No.EC 1.1.1.49
CAS No.9001-40-5
SourceZymomonas mobilis
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: