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Glutamate Dehydrogenase (NAD(P)) from E.coli, Recombinant

Cat No.
NATE-0981
Description
Glutamate dehydrogenase (GLDH) is an enzyme, present in most microbes and the mitochondria of eukaryotes, as are some of the other enzymes required for urea synthesis, that converts glutamate to α-ketoglutarate, and vice versa. In animals, the produced ammonia is usually used as a substrate in the urea cycle. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals, as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate.
Abbr
GLDH (NAD(P)), Recombinant (E.coli)
Alias
GLDH
Source
E.coli
Applications
Use recombinant Glutamate Dehydrogenase in diagnostic tests for the determination of ammonia, urea, L-glutamate, glutamate pyruvate transaminase and leucine aminopeptidase.
Appearance
White lyophilizate
Activity
>80 U/mg
CAS No.
2604152
Contaminants
Alcohol dehydrogenase: <0.005 Lactate dehydrogenase: <0.005 Malate dehydrogenase: <0.005 "NADH-Oxidase": <0.005 NH4: <0.05 μg/mg lyophilizate
Molecular Weight
~2 200 kD for the associated enzyme with 8 subunits; 280 kD for one subunit.
pH Stability
5.5-6.5
Michaelis Constant
L-glutamate: 1.8 x 10-3 mol/l NADP: 4.7 x 10-5 mol/l α-ketoglutarate: 7.0 x 10-4 mol/l NH4+: 3.2 x 10-3 mol/l NADPH: 2.6 x 10-5 mol/l Km values for NAD or NADH are difficult to obtain due to their inhibitory action.
Specificity
The oxidation of L-glutamate is stimulated by ADP and inhibited by GTP. In contrast, the oxidation of alanine, leucine, isoleucine, methionine, valine, norleucine, norvaline and 2-aminobutyrates is stimulated by GTP and inhibited by ADP.
Optimum pH
8
Activators
Thioglycolic acid, b-mercaptoethylamine, EDTA, α, α'-dipyridyl
Stability
At +2 to +8°C within specification range for 12 months. Store dry.
Inhibitors
4-chloromercuribenzoate, Na2S, diethyldithiocarbamate, 1,10-phenanthroline, 8-hydroxyquinoline, NaN3, thyroxine, heparin, sulfonylcarbamides, Cu2+, Hg2+, Ag2+, Fe3+, Zn2+, K+, PO42-, NO3-
Synonyms
glutamate dehydrogenase (NADP+); glutamic dehydrogenase; dehydrogenase; glutamate (nicotinamide adenine dinucleotide (phosphate)); glutamic acid dehydrogenase; L-glutamate dehydrogenase; L-glutamic acid dehydrogenase; NAD(P)-glutamate dehydrogenase; NAD(P)H-dependent glutamate dehydrogenase; glutamate dehydrogenase (NADP); GLDH
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