Description
Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.
Abbr
Penicillinase, Native (Bacillus cereus)
Applications
Used in the production of penicillin
Package
Bottomless glass bottle. Contents are inside inserted fused cone.
Form
Type I, lyophilized powder, white; Type II, Lyophilized powder containing phosphate and Citrate buffer salts.
Enzyme Commission Number
EC 3.5.2.6
Activity
Type I, ~13 units/mg; Type II, 1,500-3 ,000 units/mg protein (using benzylpenicillin).
Molecular Weight
Mr ~30 kDa
Unit Definition
One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25°C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Polleck units.
Warnings
Used to destroy β-lactam antibiotics in body fluids or culture media. For the determination of penicillin; In the coupled enzyme assay for isopenicillin N synthetase; ELISA of estradiol; Penicillinase optodes
Synonyms
EC 3.5.2.6; β-lactamase; penicillinase; cephalosporinase; neutrapen; penicillin β-lactamase; exopenicillinase; ampicillinase; penicillin amido-β-lactamhydrolase; penicillinase I, II; β-lactamase I-III; β-lactamase A, B, C; β-lactamase AME I; cephalosporin-β-lactamase; 9073-60-3
