Description
Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.
Abbr
Galactosyltransferase, Native (Bovine)
Applications
Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.
Form
Lyophilized powder containing Tris, EDTA, and (NH4)2SO4
Enzyme Commission Number
EC 2.4.1.22
Contaminants
<10 % protein α-lactalbumin
Unit Definition
One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30°C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)
Synonyms
EC 2.4.1.22; UDP-α-D-galactose-glucose galactosyltransferase; N-acetyllactosamine synthase; uridine diphosphogalactose-glucose galactosyltransferase; lactose synthetase; UDP-galactose:D-glucose 4-β-D-galactotransferase; UDP-galactose:D-glucose 4-β-D-galactosyltransferase; 9031-68-9
