Products

Enzymes for Research, Diagnostic and Industrial Use

Native Bovine Glutamate Dehydrogenase

Cat No.
DIA-146
Description
Glutamate dehydrogenase (GLDH) is an enzyme, present in most microbes and the mitochondria of eukaryotes, as are some of the other enzymes required for urea synthesis, that converts glutamate to α-ketoglutarate, and vice versa. In animals, the produced ammonia is usually used as a substrate in the urea cycle. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals, as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate.
Abbr
GLDH (Bovine)
Alias
GLDH
Source
Bovine liver
Species
Bovine
Appearance
White/off white powder
Form
Freeze dried powder
Enzyme Commission Number
EC 1.4.1.3
Activity
> 500U /mg protein
CAS No.
9001-46-1
Molecular Weight
260 kDa (gel)
Isoelectric point
pH5.6
pH Stability
5.0~11.0
Michaelis Constant
9.5×10-3M (NH3); 5.0×10-3M (α-Ketoglutarate); 8.4×10-5M (NADH)
Unit Definition
One unit will convert one micromole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30°C.
Optimum pH
8.5(α-KG→L-Glu)
Optimum temperature
45°C
Thermal stability
< 60°C (pH8.3, 10min)
Storage
-20°C
Inhibitors
Ag+, Hg2+, Cu2+, Zn2+ .
Preparation Instructions
The enzyme is reconstituted in 100mM Tris-HCl buffer, pH 8.3 for activity assay.
Pathway
Arginine and proline metabolism; D-Glutamine and D-glutamate metabolism; Glutamate metabolism; Nitrogen metabolism; Metabolism of amino acids.
Function
ATP binding; GTP binding; glutamate dehydrogenase [NAD(P)+] activity; glutamate dehydrogenase activity; nucleotide binding; oxidoreductase activity.
Synonyms
glutamate dehydrogenase [NAD(P)+]; EC 1.4.1.3; GLDH; glutamic dehydrogenase; glutamate dehydrogenase [NAD(P)]; L-glutamate: NAD(P)+ oxidoreductase (deaminating); L-GLDH; Glutamate Dehydrogenase from bovine liver; L-Glutamic Dehydrogenase; glutamate dehydrogenase
Download Datasheet:


“GLDH” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1701
EC No.EC 1.4.1.2
CAS No.9001-46-1
SourceE. coli
CatalogNATE-1802
EC No.EC 1.4.1.2
CAS No.9001-46-1
SourceE. coli
CatalogDIA-196
EC No.EC 1.4.1.4
CAS No.2604121
SourceProteus sp.
CatalogDIA-197
EC No.EC 1.4.1.2
CAS No.9001-46-1
SourceMicroorganism
+ See More >>
Realted Protocols
L-GLUTAMIC DEHYDROGENASE -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: