Description
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Abbr
Hyaluronidase, Native (Bovine)
Enzyme Commission Number
EC 3.2.1.35
Activity
Type I, 750-3000 units/mg solid; Type II, 300-1,000 units/mg; Type III, 3,000-15,000 units/mg solid; Type IV, 400-1000 units/mg solid.
Molecular Weight
mol wt ~55 kDa (four subunits of 14 kDa each)
Unit Definition
One unit will cause a change in A600 of 0.330 per minute at pH 5.7 at 37°C in a 2.0 ml reaction mixture (45 minute assay).
Pathway
Chondroitin sulfate degradation, organism-specific biosystem; Chondroitin sulfate degradation, conserved biosystem; Defective B4GALT1 causes B4GALT1-CDG (CDG-2d), organism-specific biosystem
Function
hyaluronan synthase activity; hyalurononglucosaminidase activity; transcription factor binding; enzyme binding; hyaluronic acid binding; hyaluronoglucuronidase activity
Synonyms
hyaluronoglucosaminidase; hyaluronidase; hyaluronoglucosidase; chondroitinase; chondroitinase I; hyaluronate 4-glycanohydrolase; EC 3.2.1.35; 37326-33-3
