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Native Bovine Phosphodiesterase, 3',5'-cyclic-nucleotide-specific

Cat No.
NATE-0515
Description
Hydrolyzes the 3',5'-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5'-monophosphate.
Abbr
PDE, Native (Bovine)
Alias
PDE
Source
Bovine brain
Species
Bovine
Applications
May be used to assay the protein activator, calmodulin.
Package
Package size based on activated units
Form
Lyophilized powder containing Tris-HCl buffer salts and lactose
Enzyme Commission Number
EC 3.1.4.17
Activity
15-30 units/mg protein (in the presence of 0.03 mM Ca2+ and a saturating level (10 units per ml) of calmodulin (P2277))
CAS No.
9040-59-9
Molecular Weight
mol wt ~60 kDa
Unit Definition
One unit will hydrolyze 1.0 μmole of 3',5'-cyclic-AMP to 5'-AMP per min at pH 7.5 at 30°C.
Storage
−20°C
Buffer
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20°C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4°C.
Warnings
Affinity purified preparation which has been depleted of calmodulin. Protein determined by Warburg-Christian
Pathway
Ca-dependent events, organism-specific biosystem; calmodulin binding; protein binding
Synonyms
cyclic 3',5'-mononucleotide phosphodiesterase; PDE; cyclic 3',5'-nucleotide phosphodiesterase; cyclic 3',5'-phosphodiesterase; 3',5'-nucleotide phosphodiesterase; 3':5'-cyclic nucleotide 5'-nucleotidohydrolase; 3',5'-cyclonucleotide phosphodiesterase; cyclic nucleotide phosphodiesterase; 3', 5'-cyclic nucleoside monophosphate phosphodiesterase; 3':5'-monophosphate phosphodiesterase (cyclic CMP); cytidine 3':5'-monophosphate phosphodiesterase (cyclic CMP); cyclic 3',5-nucleotide monophosphate phosphodiesterase; nucleoside 3',5'-cyclic phosphate diesterase; nucleoside-3',5-monophosphate phosphodiesterase; EC 3.1.4.17
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Realted Products
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ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0514
EC No.EC 3.1.4.17
CAS No.9040-59-9
SourceBovine heart
CatalogNATE-0516
EC No.EC 3.1.4.17
CAS No.9040-59-9
SourcePorcine brain
CatalogEXWM-3716
EC No.EC 3.1.4.17
CAS No.9040-59-9
Source
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