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Native Bovine Phosphodiesterase II

Cat No.
NATE-0518
Description
Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. The enzyme acts on poly (A), poly (U), and poly (I). Native DNA and poly (C) are quite resistant to the action of this enzyme. Hydrolyzes RNA, RNA-Core, 3'-alkyl-and 3'-aryl-nucleoside phosphates, and polydeoxyribonucleotides with 3'-phosphate end groups to 3'-mononucleotides. Polynucleotides having 5'-phosphomonoester end groups are not attacked.
Abbr
PDE2, Native (Bovine)
Alias
PDE2
Source
Bovine spleen
Species
Bovine
Applications
Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase II has been used in the enzymatic digestions of purified proteins such as the P8-dGMP complex. Bovine spleen phosphodiesterase has been used to digest N-cadherin. The product has been used in the characterization of polynucleotide chain length, base composition, and identity of terminal nucleotide. The enzyme has also been used in excision of pyridyloxobutyl (POB) base adducts from DNA. Furthermore, it has been used along with micr oc occal endonuclease to hydrolyze purified DNA to 3-nucleoside monophosphates.
Form
lyophilized powder
Enzyme Commission Number
EC 3.1.16.1
Activity
> 5.0 units/mg protein
CAS No.
9068-54-6
Unit Definition
One unit will produce acid soluble nucleotides equivalent to a ΔA260 of 16 in 30 min at pH 6.5 at 37°C, in a 2.0 mL reaction mixture. Substrate: RNA-Core. Actual A260 is measured on the supernatant after precipitation of the unhydrolyzed RNA with uranyl acetate-perchloric acid reagent.
Storage
−20°C
Warnings
Protein determined by biuret
Pathway
Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem
Synonyms
3'-exonuclease; spleen phosphodiesterase; 3'-nucleotide phosphodiesterase; phosphodiesterase II; spleen exonuclease; EC 3.1.16.1; 9068-54-6; PDE2
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