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Enzymes for Research, Diagnostic and Industrial Use

Native Human Topoisomerase I


Cat No.
NATE-0707
Description
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right-and left-handed superhelices.
Abbr
TOPO I, Native (Human)
Alias
TOPO I; ω-protein
Source
Human
Applications
Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.
Product Overview
Cellular topoisomerase I is present in retroviral particles and enhances viral cDNA synthesis.
Form
buffered aqueous glycerol solution; Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).
Enzyme Commission Number
EC 5.99.1.2
Activity
> 2 units/μL
CAS No.
80449-01-0
Molecular Weight
mol wt 100 kDa
Unit Definition
One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37°C.
Storage
−70°C
Pathway
Caspase cascade in apoptosis, organism-specific biosystem
Function
ATP binding; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; chromatin DNA binding; chromatin binding; nucleotide binding; protein binding
Synonyms
Topoisomerase I; EC 5.99.1.2; type I DNA topoisomerase; untwisting enzyme; relaxing enzyme; nicking-closing enzyme; swivelase; ω-protein; deoxyribonucleate topoisomerase; topoisomerase; type I DNA topoisomerase; DNA topoisomerase; TOPO I
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