Topoisomerase I from E. coli, Recombinant

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Cat No.

NATE-1339

Description

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right-and left-handed superhelices.

Abbr

TOPO I, Recombinant (E. coli)

Alias

TOPO I

Source

E. coli

Species

E. coli

Product Overview

Topoisomerase I (E. coli) catalyzes the relaxation of negatively supercoiled DNA. Topoisomerase I has also been implicated in knotting and unknotting DNA and in linking complementary rings of single-stranded DNA into double-stranded rings. The intact holoenzyme is a 97 kDa protein.

Form

50 mM KCl, 10 mM Tris-HCl (pH 7.5), 35 mM (NH4)2SO4, 0.1 mM EDTA, 1 mM DTT and 50% glycerol.

Concentration

5,000 units/ml

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the relaxation of >95% of 0.5 μg of pUC19 RF I (negatively supercoiled) DNA in 15 minutes at 37°C in a total reaction volume of 25 μl. DNA supercoiling is assessed by agarose gel electrophoresis in the absence of ethidium bromide.

Storage

at -20°C

Synonyms

Topoisomerase I; EC 5.99.1.2; type I DNA topoisomerase; untwisting enzyme; relaxing enzyme; nicking-closing enzyme; swivelase; ω-protein; deoxyribonucleate topoisomerase; topoisomerase; type I DNA topoisomerase; DNA topoisomerase; TOPO I

"TOPO I" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0707	Native Human Topoisomerase I	EC 5.99.1.2	80449-01-0	Human	Inquiry
NATE-0708	Native Vaccinia virus Topoisomerase I	EC 5.99.1.2	80449-01-0	Vaccinia virus	Inquiry
NATE-0709	Native Wheat germ Topoisomerase I	EC 5.99.1.2	80449-01-0	Wheat germ	Inquiry
NATE-1340	Topoisomerase I from Human, Recombinant			Baculovirus-infected insect cells (Spodoptera frugiperda)	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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