Native Human Topoisomerase I

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Cat No.

NATE-0707

Description

Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right-and left-handed superhelices.

Abbr

TOPO I, Native (Human)

Alias

TOPO I; ω-protein

Source

Human

Applications

Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.

Product Overview

Cellular topoisomerase I is present in retroviral particles and enhances viral cDNA synthesis.

Form

buffered aqueous glycerol solution; Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

Enzyme Commission Number

EC 5.99.1.2

Activity

> 2 units/μL

CAS_No

80449-01-0

Molecular Weight

mol wt 100 kDa

Unit Definition

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37°C.

Storage

−70°C

Pathway

Caspase cascade in apoptosis, organism-specific biosystem

Function

ATP binding; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; chromatin DNA binding; chromatin binding; nucleotide binding; protein binding

Synonyms

Topoisomerase I; EC 5.99.1.2; type I DNA topoisomerase; untwisting enzyme; relaxing enzyme; nicking-closing enzyme; swivelase; ω-protein; deoxyribonucleate topoisomerase; topoisomerase; type I DNA topoisomerase; DNA topoisomerase; TOPO I

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Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0708	Native Vaccinia virus Topoisomerase I	EC 5.99.1.2	80449-01-0	Vaccinia virus	Inquiry
NATE-0709	Native Wheat germ Topoisomerase I	EC 5.99.1.2	80449-01-0	Wheat germ	Inquiry
NATE-1339	Topoisomerase I from E. coli, Recombinant			E. coli	Inquiry
NATE-1340	Topoisomerase I from Human, Recombinant			Baculovirus-infected insect cells (Spodoptera frugiperda)	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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