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Native Klebsiella pneumoniae Pullulanase

Cat No.
NATE-0643
Description
Pullulanase is a lipoprotein generated as a precursor containing a 19-amino acid signal peptide followed by a palmitate-modified cysteine residue. The signal peptide gets cleaved prior to secretion into the extracellular matrix.
Abbr
Pullulanase, Native (Klebsiella pneumoniae)
Source
Klebsiella pneumoniae
Applications
Pullulanase has been used in a study to assess its l ocation in Escherichia coli K12 carrying the cloned structural gene from Klebsiella pneumoniae. It has also been used in a study to investigate the role of lipid modification on the enzyme in comparison to unmodified pullulanases.
Product Overview
Pullulanase is a lipoprotein generated as a precursor containing a 19-amino acid signal peptide followed by a palmitate-modified cysteine residue. The signal peptide gets cleaved prior to secretion into the extracellular matrix.
Form
Type I, Lyophilized powder containing potassium phosphate buffer salts and stabilizer; Type II, ammonium sulfate suspension, Suspension in 3.2 M (NH4)2SO4 solution, pH 6.2.
Enzyme Commission Number
EC 3.2.1.41
Activity
Type I, 10-30 units/mg protein; Type II, > 5 units/mg protein (biuret).
CAS No.
9075-68-7
Unit Definition
One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25°C.
Storage
−20°C
Synonyms
Pullulanase; EC 3.2.1.41; limit dextrinase (erroneous); amylopectin 6-glucanohydrolase; bacterial debranching enzyme; debranching enzyme; α-dextrin endo-1,6-α-glucosidase; R-enzyme; pullulan α-1,6-glucanohydrolase; 9075-68-7
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1122
EC No.EC 3.2.1.41
CAS No.9075-68-7
SourceKlebsiella planticola
CatalogNATE-1123
EC No.EC 3.2.1.41
CAS No.9075-68-7
SourceBacillus licheniformis
CatalogNATE-1227
EC No.EC 3.2.1.41
CAS No.9075-68-7
SourceBacillus subtilis subsp. subtilis str. 168
CatalogNATE-1448
EC No.EC 3.2.1.41
CAS No.9075-68-7
SourceE. coli
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Realted Protocols
PULLULANASE -Enzymatic Assay Protocol
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