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Native Pseudomonas testosteroni 3α-Hydroxysteroid Dehydrogenase


Cat No.
NATE-0007
Description
In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction:androsterone + NAD (P)+↔ 5alpha-androstane-3,17-dione + NAD (P)H + H+. The 3 substRates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases.
Abbr
3α-Hydroxysteroid Dehydrogenase, Native (Pseudomonas testosteroni)
Alias
sterognost 3α
Source
Pseudomonas testosteroni
Form
Lyophilized powder containing potassium phosphate buffer salt and EDTA
Enzyme Commission Number
EC 1.1.1.50
Activity
> 15 units/mg protein
CAS No.
9028-56-2
Unit Definition
One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25°C in the presence of β-NAD+.
Storage
−20°C
Synonyms
hydroxyprostaglandin dehydrogenase; 3α-hydroxysteroid oxidoreductase; sterognost 3α; 3α-hydroxysteroid dehydrogenase (B-specific); 3α-hydroxysteroid 3-dehydrogenase (B-specific); 3α-hydroxysteroid:NAD (P)+ 3-oxidoreductase (B-specific); EC 1.1.1.50
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