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Enzymes for Research, Diagnostic and Industrial Use

Native Rhizopus sp. Glucoamylase

Cat No.
DIA-190
Description
Glucan 1,4-alpha-glucosidase is an enzyme located on the brush border of the small intestine with system name 4-alpha-D-glucan glucohydrolase. This enzyme catalyses the following chemical reaction: Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. Most forms of the enzyme can rapidly hydrolyse 1,6-alpha-D-glycosidic bonds when the next bond in the sequence is 1,4.
Abbr
Glucoamylase (Rhizopus sp.)
Source
Rhizopus sp.
Applications
This enzyme is useful for structural investigation of carbohydrates and for enzymatic determination of α-amylase when coupled with the related enzymes in clinical analysis.
Appearance
White amorphous powder (salt-free), lyophilized
Form
Freeze dried powder
Enzyme Commission Number
EC 3.2.1.3
Activity
GradeⅠ 30U/mg-solid or more
CAS No.
9032-08-0
Molecular Weight
approx. 70 kDa
pH Stability
pH 4.0-8.5 (25°C, 20hr)
Michaelis Constant
11±1.1×10⁻⁴M (Maltose), 3.6±0.51×10⁻⁴M (Maltotriose), 2.5±0.33×10⁻⁴M (Maltotetraose), 1.6±0.02×10⁻⁴M (Maltopentaose)
Specificity
This enzyme completely hydrolyzes soluble starch, amylopectin, glycogen,α-orß-limit dextrin, amylose, maltooligosaccharides and panose.
Optimum pH
4.5-5.0
Optimum temperature
60°C
Thermal stability
below 45°C (pH 5.5, 10min)
Stability
Stable at-20°C for at least 6 months
Synonyms
EC 3.2.1.3; glucoamylase; amyloglucosidase; gamma-amylase; lysosomal alpha-glucosidase; acid maltase; exo-1,4-alpha-glucosidase; glucose amylase; gamma-1,4-glucan glucohydrolase; acid maltase; 1,4-alpha-D-glucan glucohydrolase
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0075
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceAspergillus niger
CatalogNATE-0076
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceRhizopus sp.
CatalogNATE-1051
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceAspergillus niger
CatalogNATE-1079
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceHormoconis resinae
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Realted Service
Realted Protocols
ß-N-ACETYLGLUCOSAMINIDASE -Enzymatic Assay Protocol
HYALURONIDASE -Enzymatic Assay Protocol
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