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Renin from Mouse, recombinant

Cat No.
NATE-1671
Description
Renin, also known as an angiotensinogenase, is an enzyme that participates in the body's renin-angiotensin aldosterone system (RAAS)—also known as the renin-angiotensin-aldosterone axis—that mediates extracellular volume (i.e., that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. Thus, it regulates the body's mean arterial blood pressure. Renin is often improperly referred to as a hormone even though it has no peripheral receptors and rather has an enzymatic activity with which it hydrolyses angiotensinogen to angiotensin I.
Abbr
REN, Recombinant (Mouse)
Source
HEK 293 cells
Species
Mouse
Form
Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Enzyme Commission Number
EC 3.4.23.15
Activity
>25 pmol/min/µg
CAS No.
9015-94-5
Molecular Weight
The protein is fused with 6×his tag at the C-terminus and has a calculated MW of 43.2 kDa expressed. The predicted N-terminus is Leu24. Protein migrates as 45-55 kDa in reduced SDS-PAGE due to glycosylation.
Purity
> 90% by SDS-PAGE
Storage
Store at -20°C. After reconstitution, aliquot and store at -20°C and use within 3 months. Avoid repeated freezing and thawing cycles.
Synonyms
CYMP; CYM; RENNIN; Chymosin
Endotoxin Level
< 0.1 ng/μg of protein (<1EU/μg).
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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