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Uricase from Candida utilis, Recombinant

Cat No.
DIA-404
Description
The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2
Abbr
UO, Recombinant (Candida utilis)
Source
Escherichia coli
Species
Candida utilis
Applications
Used in the enzymatic determination of uric acid.
Appearance
White to cream powder
Enzyme Commission Number
EC 1.7.3.3
Activity
> 6 U/mg
CAS No.
9002-12-4
Molecular Weight
34kDa (SDS-PAGE)
pH Stability
7.6 to 10.0
Unit Definition
One unit of activity is defined as the amount of enzyme that will transform of 1.0 micromole of substrate per minute at 25°C under standard assay method conditions.
Optimum pH
8.5
Optimum temperature
55°C
Thermal stability
Stable at 55°C and below.
Synonyms
urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II
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URICASE -Enzymatic Assay Protocol
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