Chemically modified Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

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Cat No.

DIA-280

Description

Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction:D-glucose 6-phosphate + NADP+ ↔ 6-phospho-D-glucono-1,5-lactone + NADPH + H+. This enzyme is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).

Abbr

Chemically modified G6PDH, Recombinant (Leuconostoc mesenteroides)

Alias

G6PDH; GPD

Source

E. coli

Species

Leuconostoc mesenteroides

Applications

Use Glucose-6-phosphate Dehydrogenase for the determination of blood glucose or creatine kinase.

Appearance

White lyophilizate

Activity

>30 U/mg lyophilizate

Contaminants

ATPase: <0.02 Creatine kinase: <0.001 Glutamate dehydrogenase: <0.01 Glutathione reductase: <0.001 Hexokinase and GIucose dehydrogenase: <0.05 Myokinase: <0.05 ''NADH oxidase'': <0.02 ''NADPH oxidase'': <0.0005 6-Phosphogluconate dehydrogenase: <0.001 Phophoglucose isomerase : <0.01 Phosphoglucomutase: <0.001 Glucose: <0.3 μg/mg lyophilizate

Molecular Weight

110 kD (2 identical subunits 55,000 D)

Isoelectric point

4.6

pH Stability

5.0-10.0

Michaelis Constant

NAD: 1.4 x 10-4 mmol/l NADP: 3.7 x 10-5 mmol/l Glucose-6-P: 3.7 x 10-4 mmol/l (NAD as coenzyme) Glucose-6-P: 2.0 x 10-4 mmol/l (NAD as coenzyme)

Specificity

G6P-DH is highly specific for glucose-6-phosphate and does not react with fructose-6-P, fructose-1,6-P2 or glucose-1P. 2-Deoxyglucose-6-P is slowly oxidized with NAD (5%) and with NADP (4%).

Optimum pH

7.8

Activators

Phosphate, 5 mmol/l: 100% (NAD), 80% (NADP) Phosphate, 50 mmol/l: 100% (NAD), 80% (NADP) Without Mg2+: 90% (NAD), 80% (NADP) Mg2+, 3 mmol/l: 100% (NAD), 100% (NADP) Mg2+, 30 mmol/l: 100% (NAD), 100% (NADP) HCO3-, 3 mmol/l: 100% (NAD), 100% (NADP)

Thermal stability

Up to +40°C for native G6P-DH, up to +50°C for modified G6P-DH

Stability

At +2 to +8°C within specification range for 18 months. Store dry.

Inhibitors

NADPH is a competitive inhibitor in the NAD-dependent reaction. Unlike the yeast enzyme, myristic acid, dehydroepiandrosterone and palmitoyl CoA do not inhibit.

Synonyms

D-glucose 6-phosphate dehydrogenase; glucose 6-phosphate dehydrogenase (NADP); NADP-dependent glucose 6-phosphate dehydrogenase; 6-phosphoglucose dehydrogenase; Entner-Doudoroff enzyme; glucose-6-phosphate 1-dehydrogenase; G6PDH; GPD; glucose-6-phosphate dehydrogenase

"Glucose-6-phosphate dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1898	Native Zymomonas mobilis Glucose-6-Phosphate Dehydrogenase	EC 1.1.1.49	9001-40-5	Zymomonas mobilis	Inquiry
DIA-143	Native Bacillus sp. Glucose-6-phosphate dehydrogenase	EC 1.1.1.49	9001-40-5	Bacillus sp.	Inquiry
DIA-145	Native Microorganism Glucose-6-phosphate Dehydrogenase	EC 1.1.1.49	9001-40-5	Microorganism	Inquiry
DIA-219	Native baker's yeast (S. cerevisiae) Glucose-6-phosphate Dehydrogenase	EC 1.1.1.49	9001-40-5	Baker's yeast (S. cerevisiae)	Inquiry

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